Fig. 5. Effects of Twist knockdown on ChR associated inhibition of EMT and CSLC features in HeLa cells induced by TGF-β and TNF-α co-treatment. HeLa cells expressing shTwist1 were co-administered TNF-α and TGF-β, with or without ChR(10.0μM). Then, cell morphology (A, scale bar, 50 μm), E-cadherin and N-cadherin protein amounts (B), NF-κBp65 and Twist1 protein levels (C), cell migration (D) and self-renewal (E, scale bar, 200 μm) abilities, and the protein expression levels of Bmi1, Sox2, and Oct4 (F) as well as CD133, ALDH1m and CD44(G) were evaluated. β-actinserved as an internal reference for immunoblots. GFP, cells transduced with adenoviruses carrying the GFP gene; shRNA, cells transduced with adenoviruses expressing shTwist1.^*p<0.05 vs GFP. ^#p<0.05 vs GFP with ChR(10.0μM) treatment.